Application

DNA Molecular Weight Marker XVI (250 bp ladder) may be used as a molecular weight marker in agarose gel electrophoresis: for the molecular weight comparison of random amplified polymorphic DNA (RAPD) amplified fragments of plant genomic DNA and meat productsfor enterobacterial repetitive intergenic consensus (ERIC) products generated from polymerase chain reactionin reverse transcription-polymerase chain reaction (RT-PCR) amplified dsRNA sequences of African horse sickness virus

General description

Fragment mixture prepared by cleavage of a specially constructed plasmid with restriction endonucleases.Size Range: 0.25 to 3.09 kbp

Other Notes

For life science research only. Not for use in diagnostic procedures.

Physical form

Ready-to-use solution, 250 µg/ml, in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0).

Preparation Note

Storage conditions (working solution): 2 to 8 °COnce thawed, store at 2 to 8 °C.

Sequence

The mixture contains 12 blunt-ended, double-stranded DNA fragments with the following base pair lengths (1 base pair = 660 daltons): 250, 500, 750, 1000, 1250, 1500, 1750, 2000, 2250, 2500, 2750, and 3000. The 1000- and 2000-bp banding pattern are 3 times brighter. Electrophoretic separation of this molecular weight marker results in a regular pattern.Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels. The fragments exhibit uniform spacing (e.g., on a 0.7% agarose gel) for easier reading, while bands 1000 and 2000bp are 3 times brighter for easy orientation.

Unit Definition

50 µg = 1A₂₆₀ unit